Espanyol captain Daniel Jarque has died after he suffered a cardiac arrest following a training session, the club have confirmed in a statement.
The 26-year-old midfielder had been training in Coverciano, Italy, where the squad are on a pre-season camp.
Club doctors and Italian paramedics tried to revive Jarque without success.
His death comes two years after Sevilla defender Antonio Puerta, 22, died of heart failure after collapsing during a Spanish league game.
Jarque joined Espanyol at the age of 12, making his debut in 2002, and the Spaniard was handed the club captaincy this summer.
"Tragedy struck Espanyol and the family of Dani Jarque this evening. The player died from a cardiac arrest," said a club statement.
"The doctor carried out CPR on the player and used a defibrillator, which showed that the arrest was non responsive.